Thermo Fisher Scientific Nucleolin (Marker of Human Cells) Monoclonal Antibody (SPM614)
인간 세포의 마커인 Nucleolin을 인식하는 SPM614 단클론 항체입니다. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 사용 가능하며, 인간 특이적으로 반응합니다. 액상 형태로 제공되며 4°C에서 보관합니다.
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Thermo Fisher Scientific Nucleolin (Marker of Human Cells) Monoclonal Antibody (SPM614)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–4 µg/mL |
| Flow Cytometry (Flow) | 0.5–1 µg/10^6 cells |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SPM614 |
| Immunogen | Recombinant full-length human NCL protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Does not react with Mouse, Rat, or Cow.
Target Information
Nucleolin (NCL) is a eukaryotic nucleolar phosphoprotein involved in ribosome synthesis and maturation. It is mainly located in the dense fibrillar regions of the nucleolus. The human NCL gene consists of 14 exons and 13 introns spanning approximately 11 kb. Intron 11 encodes a small nucleolar RNA termed U20.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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