Thermo Fisher Scientific Nucleolin (Marker of Human Cells) Monoclonal Antibody (364-5)
Nucleolin 단백질을 표적하는 인간 세포 마커용 단클론 항체. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합. 사람 시료에 반응하며, 마우스·랫·소에는 반응하지 않음. 단백질 A/G 정제, PBS 용액 형태로 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 0.25–0.5 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 364-5 |
| Immunogen | Lysate of SU-DHL-1 nuclei |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Does not react with Mouse, Rat, or Cow.
Target Information
Nucleolin (NCL) is a eukaryotic nucleolar phosphoprotein involved in the synthesis and maturation of ribosomes. It is primarily located in the dense fibrillar regions of the nucleolus.
The human NCL gene comprises 14 exons and 13 introns, spanning approximately 11 kb. Intron 11 encodes a small nucleolar RNA known as U20.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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