Thermo Fisher Scientific Nucleolin (Marker of Human Cells) Monoclonal Antibody (364-5, NCL/902)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1-2 µg/mL

-

Immunocytochemistry (ICC/IF)

0.5-1 µg/mL

-

Flow Cytometry (Flow)

0.5-1 µg/10^6 cells

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

364-5, NCL/902

Immunogen

Lysate of SU-DHL-1 Nuclei (364-5). Recombinant human NCL protein (NCL/902) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Nucleolin,uniProtId:P19338-1,ncbiNodeId:9606,antigenRange:1-710,antigenLength:710,antigenImageFileName:4691-MSM3-P0_Nucleolin_P19338-1_House_mouse.svg,antigenImageFileNamePDP:4691-MSM3-P0_Nucleolin_P19338-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

200 µg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Does not react with Mouse, Rat, Cow

Target Information

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.