Thermo Fisher Scientific Nucleolin (Marker of Human Cells) Monoclonal Antibody (364-5, NCL/902)
인간 세포 마커 Nucleolin을 인식하는 mouse monoclonal antibody로, WB, IHC, ICC, Flow 등 다양한 응용 가능. Human에 반응하며 Mouse, Rat, Cow에는 반응하지 않음. Protein A/G 정제, 액상 형태, 4°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.5–1 µg/mL |
| Flow Cytometry (Flow) | 0.5–1 µg/10^6 cells |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 364-5, NCL/902 |
| Immunogen | Lysate of SU-DHL-1 Nuclei (364-5); Recombinant human NCL protein (NCL/902) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Does not react with Mouse, Rat, Cow.
Target Information
Nucleolin (NCL) is a eukaryotic nucleolar phosphoprotein involved in ribosome synthesis and maturation. It is primarily located in the dense fibrillar regions of the nucleolus. The human NCL gene contains 14 exons and 13 introns, spanning approximately 11 kb. Intron 11 encodes a small nucleolar RNA, termed U20.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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