Thermo Fisher Scientific Nucleolin (Marker of Human Cells) Monoclonal Antibody (364-5)
인간 세포의 Nucleolin을 표지하는 Mouse IgG1 단일클론 항체로, WB, IHC, ICC, Flow 등 다양한 응용에 사용 가능. Protein A/G 정제, 200 µg/mL 농도, PBS 버퍼에 보존. Mouse, Rat, Cow에는 반응하지 않음.
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Thermo Fisher Scientific Nucleolin (Marker of Human Cells) Monoclonal Antibody (364-5)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 0.25–0.5 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 364-5 |
| Immunogen | Lysate of SU-DHL-1 nuclei (target family: Nucleolin, UniProt ID: P19338-1, antigen range: 1–710 aa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Does not react with Mouse, Rat, or Cow.
Target Information
Nucleolin (NCL) is a eukaryotic nucleolar phosphoprotein involved in the synthesis and maturation of ribosomes. It is primarily located in dense fibrillar regions of the nucleolus. The human NCL gene consists of 14 exons and 13 introns, spanning approximately 11 kb. Intron 11 encodes a small nucleolar RNA, termed U20.
Usage Notes
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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