Thermo Fisher Scientific CD8a Monoclonal Antibody (OX8), PE, eBioscience
Rat CD8α 인식 단클론 항체(OX8)로, PE 형광으로 표지되어 Flow Cytometry에 적합합니다. MHC I 제한 T 세포 및 대식세포 분석에 사용되며, 0.125 µg/test로 최적화되었습니다. 4°C 암소 보관, 동결 금지.
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Applications
- Flow Cytometry (Flow)
Tested dilution: 0.125 µg/test
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Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Published Species | Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OX8 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1210759 |
Additional Available Formats
- Unconjugated
- Alexa Fluor 700
- APC
- eFluor 450
- FITC
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 780
- Request custom conjugation
Product Specific Information
Description:
The OX8 monoclonal antibody reacts with rat CD8 alpha, a member of the immunoglobulin superfamily. CD8α heterodimerizes with CD8β to form the CD8 antigen expressed on CD4+CD8+ thymocytes, most MHC class I-restricted T cells, and some macrophages. CD8 interacts with MHC class I on antigen-presenting cells and participates in T-cell receptor signal transduction via the kinase Lck.
Applications Reported:
Reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of rat splenocytes. Recommended ≤0.125 µg per test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test. Optimize by titration for best performance.
Excitation / Emission:
488–561 nm / 578 nm
Laser Compatibility: Blue, Green, Yellow-Green Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein of the immunoglobulin receptor family involved in signal transduction, T-cell differentiation, and activation. CD8 functions as a co-receptor with T-cell receptor (TCR) for MHC class I molecules, while CD4 serves as a co-receptor for MHC class II. CD8α is essential for MHC-I binding and is expressed on subsets of T cells, NK cells, monocytes, and dendritic cells. Engagement with MHC-I/peptide complexes recruits Lck kinase, leading to lymphokine production and cytotoxic T lymphocyte activation. Once activated, CTLs are critical for pathogen and tumor clearance. Differentiation of naive CD8+ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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