Thermo Fisher Scientific CD8a Monoclonal Antibody (OX8), PE, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested dilution: 0.125 µg/test
  View 6 publications

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• eFluor 450
• FITC
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 780
• Request custom conjugation

Product Specific Information

Description:
The OX8 monoclonal antibody reacts with rat CD8 alpha, a member of the immunoglobulin superfamily. CD8α heterodimerizes with CD8β to form the CD8 antigen expressed on CD4+CD8+ thymocytes, most MHC class I-restricted T cells, and some macrophages. CD8 interacts with MHC class I on antigen-presenting cells and participates in T-cell receptor signal transduction via the kinase Lck.

Applications Reported:
Reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of rat splenocytes. Recommended ≤0.125 µg per test (100 µL final volume). Cell number: 10⁵–10⁸ cells/test. Optimize by titration for best performance.

Excitation / Emission:
488–561 nm / 578 nm
Laser Compatibility: Blue, Green, Yellow-Green Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein of the immunoglobulin receptor family involved in signal transduction, T-cell differentiation, and activation. CD8 functions as a co-receptor with T-cell receptor (TCR) for MHC class I molecules, while CD4 serves as a co-receptor for MHC class II. CD8α is essential for MHC-I binding and is expressed on subsets of T cells, NK cells, monocytes, and dendritic cells. Engagement with MHC-I/peptide complexes recruits Lck kinase, leading to lymphokine production and cytotoxic T lymphocyte activation. Once activated, CTLs are critical for pathogen and tumor clearance. Differentiation of naive CD8+ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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