Thermo Fisher Scientific OCT4 Polyclonal Antibody
OCT4 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, IHC, ICC/IF, Flow Cytometry에 사용 가능. 다양한 종(인간, 마우스, 랫 등)에 반응하며, 줄기세포 연구 및 발달 생물학 연구에 적합. 항원 친화 크로마토그래피로 정제된 고품질 항체.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500 | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200 | — |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | View 1 publication |
| Flow Cytometry (Flow) | 1:50–1:200 | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Non-human primate, Rat |
| Published Species | Bovine, Cat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide between amino acids 100–200 of the human OCT4 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2268556 |
Product Specific Information
This antibody is expected to react with swine, feline, and bovine samples based on a 93% sequence homology.
Target Information
POU5F1 (also known as Oct-4) is a maternally expressed octamer-binding transcription factor crucial for early embryonic development.
It plays a key role in maintaining pluripotency and self-renewal in embryonic stem cells.
POU5F1, along with Sox2, Klf4, Lin28, and c-Myc, can induce pluripotent stem (iPS) cells from somatic cells, providing a foundation for regenerative medicine research without the need for viral vectors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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