Thermo Fisher Scientific Histone H3 Polyclonal Antibody
Western blot 및 ChIP assay에 적합한 Histone H3 폴리클로날 항체. 인간 및 마우스에 반응하며, 다양한 포유류 종에서 교차 반응 가능. 합성 펩타이드(aa 100–135)로부터 유래된 면역원 사용. PBS 버퍼에 0.02% sodium azide 포함, -20°C 보관.
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Applications
Western Blot (WB)
- Tested dilution: 1:500–1:1,000
- View 1 publication
ChIP assay (ChIP)
- Tested dilution: Assay-dependent
- View references
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide between amino acids 100–135 of the human Histone H3 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_794480 |
Product Specific Information
This antibody is expected to cross-react with all mammals and a wide range of other species based on sequence homology.
Suggested positive control: HeLa whole cell lysate, MEF and HeLa lysates, MEF whole cell lysate.
Target Information
Histone H3 is a DNA-binding protein found in the chromatin of all eukaryotic cells. Along with other core histones, it binds DNA to form nucleosomes, playing a key role in transcription regulation, DNA repair, replication, and chromosomal stability.
The N-terminal tail of histone H3 can undergo various post-translational modifications—such as methylation, acetylation, and phosphorylation—that influence chromatin structure and gene expression.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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