Thermo Fisher Scientific HP1 alpha Polyclonal Antibody
HP1 alpha 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot, ICC/IF, Flow Cytometry에 사용 가능. 인간에 반응하며 마우스 등과 교차 반응 예상. 고순도 IgG 분획, PBS buffer에 0.02% sodium azide 포함. 4°C 또는 -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:500 |
| Flow Cytometry (Flow) | 1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide between amino acids 1–100 of the human HP1 alpha protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 13 mg/mL |
| Purification | IgG fraction |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2244131 |
Product Specific Information
This antibody is expected to cross-react with mouse, Arabidopsis suecica, rye, and Triticum aestivum based on sequence homology.
Suggested positive control: antigen standard for CBX5 (transient overexpression lysate), HeLa whole cell extract.
Target Information
Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing. Mammalian histone methyltransferases that methylate histone H3 on lysine-9 generate a binding site for HP1 proteins, which act as adaptor molecules in gene silencing and chromatin structure regulation. The HP1 chromodomain specifically recognizes methylated histone H3 lysine-9. In Suv39h double-knockout mouse fibroblasts, HP1 association with heterochromatin is lost but restored upon reintroduction of active SUV39H1 HMTase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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