Thermo Fisher Scientific Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 755
DyLight 755 형광으로 표지된 Donkey anti-Goat IgG(H+L) 2차 항체로, Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합합니다. 교차흡착 처리로 비특이적 반응을 최소화하였으며, 높은 특이성과 감도를 제공합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (IHC) | 1:50–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:500 |
| Flow Cytometry (Flow) | 1:50–1:200 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Goat |
| Host / Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Goat IgG-heavy and light chain |
| Conjugate | DyLight™ 755 |
| Excitation / Emission Max | 760 / 789 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2556671 |
| Target | IgG |
| Cross Adsorption | Against chicken, human, mouse, pig, rabbit, and rat |
| Antibody Form | Whole Antibody |
Product Specific Information
This antibody is cross-adsorbed and exhibits minimal reactivity to chicken, human, mouse, pig, rabbit, and rat.
Target Information
Anti-Goat secondary antibodies are affinity-purified antibodies with well-characterized specificity for goat immunoglobulins. They are useful for detection, sorting, or purification of specific targets.
Secondary antibodies provide versatility for various detection systems (e.g., HRP, AP, fluorescence) and enhance sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and may be further purified or modified (e.g., immunoaffinity purification, fragmentation, label conjugation) to achieve high specificity.
Additional Formats Available
- Alexa Fluor 350, 488, 546, 555, 568, 594, 633, 647, 680
- AP, Biotin, DSB-X Biotin
- DyLight 350, 488, 550, 594, 650, 680, 800
- FITC, HRP, TRITC
- Custom conjugation available upon request
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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