Thermo Fisher Scientific STAT4 Monoclonal Antibody (OTI2G5), TrueMAB

Applications and Tested Dilutions

Product Specifications

Additional Formats

• Biotin
• Request custom conjugation

Product Specific Information

For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, it is recommended to perform an additional desalting step using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).

Target Information

STAT4 was originally identified using degenerate primers complementary to conserved regions of other STAT proteins.
It shares 52% similarity with STAT1 and 47% with STAT3. STAT4 can be tyrosine phosphorylated by Jak1 or Jak2, forming homo- or heterodimers with other STAT family members.
Phosphorylated STAT4 binds the IFN-gamma activated site (GAS), and serine phosphorylation is required for maximal transcriptional activity.

STAT4 expression is mainly found in the thymus, spleen, and testis. It is activated by interleukin-12 (IL-12), which is essential for T-cell independent induction of IFN-gamma and Th1 response development.
STAT4-deficient mice show impaired IL-12-driven Th1 differentiation and enhanced Th2 development.
In viral infections, IFN-α/β activation of STAT4 is required for IFN-γ production.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.