Thermo Fisher Scientific CD120b (TNF Receptor II) Monoclonal Antibody (TR75-54), eBioscience
마우스 TNF Receptor II(CD120b)를 인식하는 단클론 항체로, Flow Cytometry, WB, ELISA, IP 등 다양한 분석에 사용 가능. Affinity chromatography로 정제되었으며, 4°C 보관. 연구용으로만 사용.
- 카탈로그번호
- 14-1203-8x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD120b (TNF Receptor II) Monoclonal Antibody (TR75-54), eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-Dependent |
| Flow Cytometry (Flow) | Assay-Dependent |
| ELISA | 1–4 µg/mL |
| Immunoprecipitation (IP) | Assay-Dependent |
| Neutralization (Neu) | - |
| Functional Assay | Assay-Dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | TR75-54 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1257205 |
Product Specific Information
Description:
The TR75-54 monoclonal antibody reacts with mouse Tumor Necrosis Factor Receptor II (TNFRII, TNFR-p80, TNFRSF1B, CD120b). TNFRII is expressed in various cell types and is strongly expressed on stimulated T and B lymphocytes. It is the main TNF receptor found on circulating T cells and mediates autoregulatory apoptosis in CD8+ cells. Soluble TNF receptors, truncated forms of the surface receptors, have neutralizing activity on both TNF-alpha and TNF-beta. TNF’s proinflammatory and immunosuppressive properties are believed to segregate at the receptor level, with soluble receptor-bound TNF acting as a reservoir to regulate TNF release.
Applications Reported:
This antibody has been reported for use in flow cytometry, immunoprecipitation, Western blot, and ELISA.
Applications Tested:
The TR75-54 antibody has been validated as a capture antibody in sandwich ELISA for mouse TNFRII detection when paired with the biotin-conjugated TR75-32 antibody (Product #13-1204) and recombinant mouse TNFRII as the standard. Recommended capture concentration is 1–4 µg/mL.
A standard curve using serial dilutions of recombinant standard (1000 pg/mL to 8 pg/mL) should be included in each ELISA plate.
Purity: Greater than 90%, determined by SDS-PAGE.
Aggregation: Less than 10%, determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
TNFR2 (tumor necrosis factor receptor superfamily member 1B) is a high-affinity receptor for TNFSF2/TNF-alpha and has lower affinity for TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. TNFR2 mediates most metabolic effects of TNF-alpha.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.