Thermo Fisher Scientific IL-20RB Monoclonal Antibody (20RNTC), eBioscience

Applications and Tested Dilution

Product Specifications

Product Specific Information

This 20RNTC monoclonal antibody recognizes mouse and human IL-20R2 (interleukin-20 receptor 2, IL-20Rb) in either of its two heterodimeric complexes formed by pairing with IL-20R1 or IL-22R1.
IL-20R2 belongs to the class II cytokine receptor family, characterized by conserved cysteine, proline, and tryptophan residues in the extracellular domain.

• Receptor Complexes:
  • IL-20R2 + IL-20R1: binds IL-20, IL-19, or IL-24
  • IL-20R2 + IL-22R1: binds IL-20 or IL-24

The IL-20R complex signals primarily via the JAK-STAT pathway.
IL-20R2 is mainly expressed on epithelial cells (e.g., gut, skin) and can be upregulated during inflammation.
Immunofluorescent staining of A375 cells shows punctate membrane staining and diffuse cytoplasmic staining, likely associated with protein synthesis and transport in the endoplasmic reticulum and Golgi.

• Applications Reported: Immunocytochemistry, Western Blot, Flow Cytometry
• Applications Tested: Immunofluorescent staining and flow cytometric analysis of fixed and permeabilized A375 cells (1–10 µg/mL)
• Purity: >90% by SDS-PAGE
• Aggregation: <10% by HPLC
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

IL20RB and IL20RA form a heterodimeric receptor for interleukin-20 (IL20) (Blumberg et al., 2001 [PubMed 11163236]).
[supplied by OMIM, Feb 2009]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.