Thermo Fisher Scientific IL-20RB Monoclonal Antibody (20RNTC), eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

Assay-Dependent

-

Flow Cytometry (Flow)

1-10 µg/mL

View 1 publication 1 publication

Neutralization (Neu)

-

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

20RNTC

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2864980

Product Specific Information

Description: This 20RNTC monoclonal antibody recognizes mouse and human IL-20R2 (interleukin-20 receptor 2, IL-20Rb) in either of its two heterodimeric complexes formed by pairing with IL-20R1 or IL-22R1. IL-20R2 belongs to the class II cytokine receptor family that shares a characteristic pattern of cysteine, proline and tryptophan residues in the extracellular domain. IL-20R2 paired with IL-20R1 forms a receptor that binds IL-20, IL-19, or IL-24. IL-20R2 paired with IL22R1 forms a receptor that binds IL-20 or IL-24. The IL-20R complex signals primarily via the JAK-STAT pathway. IL-20R2 is found predominantly on cells of epithelial origin such as gut and skin and can be upregulated during inflammation. Immunofluorescent staining of A375 cells reveals a punctate staining pattern at the cell membrane, as well as diffuse, cytoplasmic staining that is likely associated with protein synthesis and transport in the endoplasmic reticulum and golgi.

Applications Reported: This 20RNTC antibody has been reported for use in immunocytochemistry, immunoblotting (WB) and flow cytometric analysis.

Applications Tested: This 20RNTC antibody has been tested by immunofluorescent staining and flow cytometric analysis of fixed and permeabilized A375 cells. This can be used between 1-10 µg/mL.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL20RB and IL20RA (MIM 605620) form a heterodimeric receptor for interleukin-20 (IL20; MIM 605619) (Blumberg et al., 2001 [PubMed 11163236]).[supplied by OMIM, Feb 2009].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.