Thermo Fisher Scientific CD122 Monoclonal Antibody (TM-b1 (TM-beta1)), eBioscience

Applications and Tested Dilution

Product Specifications

Additional Available Formats

• APC
• Biotin
• eFluor 450
• FITC
• Functional Grade
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Request custom conjugation available

Product Specific Information

The TM-b1 monoclonal antibody reacts with mouse CD122, the 90–110 kDa interleukin-2 receptor beta chain (IL-2Rβ). CD122 is expressed by NK cells and some T cells, and is upregulated upon activation. It associates with CD25 and CD132 (common gamma chain) to form the high-affinity IL-2 receptor. Binding of IL-2 to its receptor is inhibited by TM-b1 antibody.

Applications Reported: Flow cytometric analysis, cytokine neutralization, and immunoprecipitation.
Applications Tested: Flow cytometric analysis of mouse splenocytes at ≤0.5 µg/test (in 100 µL final volume). Optimal antibody titration recommended.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD122 (Interleukin-2 receptor beta chain) is involved in T cell-mediated immune responses and is primarily expressed in the hematopoietic system. It exists in three affinity forms:

• Low affinity: alpha subunit monomer (no signal transduction)
• Intermediate affinity: alpha/beta heterodimer
• High affinity: alpha/beta/gamma heterotrimer

The intermediate and high affinity forms mediate receptor endocytosis and mitogenic signaling. The CD122 gene encodes the beta subunit, a type I membrane protein. Dysfunction in CD122 is associated with oligoarticular juvenile idiopathic arthritis and rheumatoid factor-negative juvenile idiopathic arthritis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.