Thermo Fisher Scientific CD8 alpha Monoclonal Antibody (5H10), PE

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-dependent

View 14 publications 14 publications

ELISA (ELISA)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b

Class

Monoclonal

Type

Antibody

Clone

5H10

Immunogen

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View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

PE PE PE

View additional formats

• Alexa Fluor 488
• Alexa Fluor 647
• Alexa Fluor 700
• APC
• APC-Alexa Fluor 750
• FITC
• Pacific Blue
• Pacific Orange
• PE-Cyanine7
• PE-Texas Red
• PerCP
• Request custom conjugation

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Purification

Affinity chromatography

Storage buffer

PBS with BSA

Contains

0.1% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10373415

Target Information

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.