Thermo Fisher Scientific CSF2RB Chimeric Recombinant Mouse Monoclonal Antibody (BION-1)

Applications and Tested Dilution

Product Specifications

Product Specific Information

Specificity:
Binds to domain 4 of the common beta-chain and recognizes the same binding site used by IL-5, GM-CSF, and IL-3, or binds to an epitope in close proximity. Residues M363, R364, E366 (B-C loop) and R418 (F-G loop) in the common beta-chain are important for BION-1 binding.

Preparation:
BALB/c mice were immunized intraperitonally with COS cells transfected DQP (Domain 4 of the common beta-chain) expression constructs. Immunizations were repeated four times at 2-week intervals. Four weeks after the final immunization, a mouse was boosted intravenously with COS transfectants. Three days later, splenocytes were harvested and fused with NS-1 myeloma cells. Hybridoma supernatants were screened on CHO DQP cells by flow cytometry, using untransfected CHO cells as controls. All antibodies were derived from single hybridoma clones selected by limiting dilution. Monoclonal antibodies were purified from ascites fluid or hybridoma supernatant by Protein A Sepharose chromatography.

Target Information

The encoded protein is the common beta chain of the high-affinity receptor for IL-3, IL-5, and CSF. Defects in this gene have been associated with pulmonary alveolar proteinosis (PAP).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.