Thermo Fisher Scientific Catenin, beta (p120) Recombinant Mouse Monoclonal Antibody (rCTNNB1, 1507)

Applications and Tested Dilution

Product Specifications

Product Specific Information

For immunohistochemistry on PFA-fixed tissues: incubate antibody for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

Beta-catenin is an adherens junction (AJ) protein originally identified as a component of cell-cell adhesion structures. AJs are essential for the formation and maintenance of epithelial cell layers by regulating cell growth and adhesion. Beta-catenin interacts with the cytoplasmic domain of E-cadherin, linking it to alpha-catenin, which anchors the E-cadherin complex to the actin cytoskeleton. It also associates with complexes containing adenomatous polyposis coli protein and microtubules, and participates in multiple signaling pathways, including Wnt/Wingless, tyrosine kinases, and phosphatases. These interactions influence morphogenesis and cell proliferation. Beta-catenin is expressed in hair follicle cells, basal and peripheral matrix cells, and root sheath cells. Pathologically, it has been implicated in pilomatrixoma, medulloblastoma, colorectal and ovarian cancers, and other solid tumors. In the nucleus, beta-catenin co-activates Lef/Tcf transcription factors, promoting expression of genes such as cyclin D and c-myc that drive proliferation and tumorigenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.