Thermo Fisher Scientific Catenin, beta (p120) Recombinant Mouse Monoclonal Antibody (rCTNNB1, 1507)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1–2 µg/mL

Flow Cytometry (Flow)

• Tested Dilution: 0.5–1 µg/10^6 cells

Product Specifications

Product Specific Information

• For IHC (PFA fixed): Incubate antibody for 30 minutes at room temperature.
• Staining of formalin-fixed tissues requires heating sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.

Target Information

Beta-catenin is an adherens junction (AJ) protein involved in cell-cell adhesion and epithelial layer maintenance. It interacts with E-cadherin and alpha-catenin to anchor the E-cadherin complex to the actin cytoskeleton. Beta-catenin also associates with the adenomatous polyposis coli protein and microtubules, participating in signaling pathways involving tyrosine kinases, phosphatases, and Wnt/Wingless.

It is expressed in various hair follicle cell types and epithelial cells. Pathological roles of beta-catenin have been identified in pilomatrixoma, medulloblastoma, colorectal cancer, ovarian cancer, and other tumors. In the nucleus, beta-catenin co-activates Lef/Tcf transcription factors, promoting expression of genes such as cyclin D and c-myc, which drive cell proliferation and tumorigenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.