
Thermo Fisher Scientific ATP5A1 Polyclonal Antibody
ATP5A1 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, IP 등 다양한 응용 가능. 인간 ATP synthase α-subunit(175–225aa) 기반 면역원 사용. 1 mg/mL 액상 제품으로 4°C 보관, Wet ice 배송. FFPE 조직은 citrate buffer(pH 6.0) 항원 복원 권장.
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Thermo Fisher Scientific ATP5A1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:2,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:2,000–1:10,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 175 to 225 of human ATP synthase subunit alpha, mitochondrial |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Rat, X. laevis, Bovine, Pig, Orangutan, and Chimpanzee
Target Information
This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation.
ATP synthase is composed of two linked multi-subunit complexes:
- F1: soluble catalytic core
- Fo: membrane-spanning proton channel component
The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and one each of the other three.
The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core.
Alternatively spliced transcript variants encoding different isoforms have been identified.
Pseudogenes of this gene are located on chromosomes 9, 2, and 16.
For Research Use Only. Not for use in diagnostic procedures.
Not for resale without express authorization.
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