Thermo Fisher Scientific ATP5A1 Polyclonal Antibody
ATP5A1 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체로, Western blot, IHC, IP 등 다양한 응용에 적합합니다. 인간 및 생쥐 반응성이 있으며, 항원 친화 크로마토그래피로 정제된 액상 형태입니다. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific ATP5A1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:2,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:2,000–1:10,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 100–150 of human ATP synthase subunit alpha, mitochondrial |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Rat, D. melanogaster, Bovine, Pig, Orangutan, and Chimpanzee
Target Information
This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis using the electrochemical gradient of protons across the inner membrane during oxidative phosphorylation.
ATP synthase consists of two linked multi-subunit complexes:
- F1: soluble catalytic core
- Fo: membrane-spanning component comprising the proton channel
The catalytic portion of mitochondrial ATP synthase contains five different subunits (alpha, beta, gamma, delta, epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and one of each of the other three.
The proton channel consists of three main subunits (a, b, c).
This gene encodes the alpha subunit of the catalytic core.
Alternatively spliced transcript variants encoding different isoforms have been identified.
Pseudogenes are located on chromosomes 9, 2, and 16.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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