Thermo Fisher Scientific CD14 Monoclonal Antibody (61D3), Super Bright 702, eBioscience
인간 CD14 단백질을 인식하는 61D3 클론의 마우스 단클론 항체로, Super Bright 702 형광체로 결합되어 있습니다. Flow cytometry에 최적화되어 있으며, 405 nm 레이저로 여기되고 702 nm에서 방출됩니다. 고감도 분석용으로 사용되며, 광산화에 민감하므로 암조건에서 보관이 필요합니다.
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Thermo Fisher Scientific CD14 Monoclonal Antibody (61D3), Super Bright 702, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 5 µL (0.25 µg)/test | View 23 publications |
| Neutralization (Neu) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Published Species | Not Applicable |
| Host/Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright™ 702, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 61D3 |
| Conjugate | Super Bright™ 702 |
| Excitation/Emission Max | 413/702 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_2662453 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV and Brilliant Violet series (395–805)
- eFluor 450, 506
- FITC
- Functional Grade
- NovaFluor Blue and Yellow series
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 780
- Custom conjugation available
Product Specific Information
The 61D3 monoclonal antibody reacts with human CD14, a 53–55 kDa GPI-linked glycoprotein expressed on monocytes, macrophages, and some dendritic cells. LPS-LBP complexes bind to monocytes via CD14 with high affinity.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-titrated for flow cytometry using normal human peripheral blood cells at 5 µL (0.25 µg)/test
- Excitation/Emission: 405 nm / 702 nm (Violet Laser)
- Recommended Filter: 710/50 bandpass
Super Bright 702 is a tandem dye excited by the violet laser and emits at 702 nm. Use Super Bright Staining Buffer (Product # SB-4400) when combining multiple Super Bright dyes to minimize non-specific interactions.
Light Sensitivity: Protect from light to avoid photo-induced oxidation.
Fixation: Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333).
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD14 is a 55 kDa GPI-anchored glycoprotein expressed on monocytes, macrophages, and neutrophils. It functions as a lipopolysaccharide receptor and is released into serum in soluble form. CD14 interacts with LPS-binding protein (LBP) and plays a role in immune responses, apoptosis, and inflammation. Dysfunctions are associated with infections such as Mycobacterium chelonae and Croup.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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