Thermo Fisher Scientific CD14 Monoclonal Antibody (61D3), Super Bright 702, eBioscience

Thermo Fisher Scientific CD14 Monoclonal Antibody (61D3), Super Bright 702, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV and Brilliant Violet series (395–805)
• eFluor 450, 506
• FITC
• Functional Grade
• NovaFluor Blue and Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 780
• Custom conjugation available

Product Specific Information

The 61D3 monoclonal antibody reacts with human CD14, a 53–55 kDa GPI-linked glycoprotein expressed on monocytes, macrophages, and some dendritic cells. LPS-LBP complexes bind to monocytes via CD14 with high affinity.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated for flow cytometry using normal human peripheral blood cells at 5 µL (0.25 µg)/test
• Excitation/Emission: 405 nm / 702 nm (Violet Laser)
• Recommended Filter: 710/50 bandpass

Super Bright 702 is a tandem dye excited by the violet laser and emits at 702 nm. Use Super Bright Staining Buffer (Product # SB-4400) when combining multiple Super Bright dyes to minimize non-specific interactions.

Light Sensitivity: Protect from light to avoid photo-induced oxidation.
Fixation: Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333).

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD14 is a 55 kDa GPI-anchored glycoprotein expressed on monocytes, macrophages, and neutrophils. It functions as a lipopolysaccharide receptor and is released into serum in soluble form. CD14 interacts with LPS-binding protein (LBP) and plays a role in immune responses, apoptosis, and inflammation. Dysfunctions are associated with infections such as Mycobacterium chelonae and Croup.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지