Thermo Fisher Scientific CD16 Monoclonal Antibody (3G8), Super Bright 702, eBioscience
인간 및 비인간 영장류 CD16(FcγRIII)에 특이적인 3G8 단클론 항체로, Super Bright 702 형광체로 결합되어 흐름세포분석(Flow Cytometry)에 최적화됨. 405nm 레이저에서 여기되어 702nm에서 방출되며, ADCC 및 면역복합체 분석 연구에 활용 가능. 빛에 민감하므로 암조건에서 보관.
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Thermo Fisher Scientific CD16 Monoclonal Antibody (3G8), Super Bright 702, eBioscience™
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (0.125 µg)/test
Publications: View 1 publication
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Baboon, Chimpanzee, Cynomolgus monkey, Human, Non-human primate, Rhesus monkey |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright™ 702 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3G8 |
| Conjugate | Super Bright™ 702 |
| Excitation / Emission Max | 413 / 702 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2662480 |
Additional Formats
- APC-eFluor 780
- Functional Grade
- NovaFluor Blue 510, 530, 585, 610-70S, 660-120S, 660-40S, 760
- NovaFluor Red 685, 700, 725, 755
- NovaFluor Yellow 590, 610, 660, 690, 700, 730, 755
- PerCP-eFluor 710
- Super Bright 436, 600, 645
- Request custom conjugation
Product Specific Information
This 3G8 monoclonal antibody reacts with human and non-human primate CD16, also known as the low-affinity Fc gamma RIII. CD16 exists as two isoforms: FcγRIIIA and FcγRIIIB.
- FcγRIIIA: Expressed on monocytes, macrophages, and NK cells
- FcγRIIIB: Detected on neutrophils
Expression patterns vary among primate species. Binding of IgG triggers antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, cytokine release, and antigen presentation.
Cross-blocking studies: 3G8 recognizes the same epitope as CB16 but distinct from B73.1.
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated for flow cytometry of human and rhesus peripheral blood cells at 5 µL (0.125 µg) per test (100 µL total volume).
Super Bright 702 is a tandem dye excited at 405 nm and emits at 702 nm. Recommended filter: 710/50 bandpass.
Use Super Bright Complete Staining Buffer (Product # SB-4401) when combining multiple Super Bright dyes to minimize non-specific interactions.
Light Sensitivity: Protect from light.
Fixation: Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333).
Laser: Violet (405 nm)
Emission: 702 nm
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD16 encodes a receptor recognizing the Fc portion of IgG, involved in immune complex clearance and antibody-dependent cytotoxicity.
- Gene: FCGR3A (chromosome 1)
- Expression: NK cells (transmembrane form), neutrophils (PI-anchored form via FCGR3B)
- Associated Diseases: Immunodeficiency 20, Herpes Zoster, systemic lupus erythematosus, neonatal neutropenia
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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