Thermo Fisher Scientific Rubicon Monoclonal Antibody (4F5B5)
Rubicon 단백질을 인식하는 마우스 단클론 항체로, Western blot과 ELISA에 적합합니다. 정제된 재조합 인간 RUBCN 단백질(AA 859-972)을 면역원으로 사용하였으며, Protein G로 정제되었습니다. 연구용으로만 사용 가능합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| ELISA | 1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4F5B5 |
| Immunogen | Purified recombinant fragment of human RUBCN (AA: 859–972) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3091029 |
Target Information
Two Beclin-1-interacting proteins, the run domain Beclin-1 interacting and cysteine-rich containing protein (Rubicon) and ATG14L, reciprocally regulate autophagy at different stages. Knockdown of Rubicon enhances autophagy, whereas knockdown of ATG14L causes a defect in autophagosome formation. Rubicon functions as part of a Beclin-1–PIK3C3-containing autophagy complex and is also an essential positive regulator of the NADPH oxidase complex. Upon microbial infection or TLR2 activation, Rubicon interacts with the CYBA subunit of the NADPH oxidase complex, leading to a burst of reactive oxygen species and inflammatory cytokines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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