Thermo Fisher Scientific WTAP Monoclonal Antibody (6B6B8)
WTAP 단백질을 인식하는 Mouse monoclonal antibody(클론 6B6B8). Western blot, IHC, Flow cytometry, ELISA에 사용 가능. Human 시료 반응성. Protein G 정제, PBS buffer(0.05% sodium azide) 포함. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | |
| Flow Cytometry (Flow) | 1:200–1:400 | |
| ELISA | 1:10,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6B6B8 |
| Immunogen | Purified recombinant fragment of human WTAP (AA: 91–201) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3090906 |
Target Information
Wilms' tumor (WT) is an embryonal malignancy of the kidney that affects approximately 1 in 10,000 infants and occurs in both sporadic and inherited forms.
The Wilms' tumor protein (WT1) binds the DNA sequence GCGGGGGCG, a recognition element common to the early growth response (Egr) family of Zn²⁺ finger transcriptional activators, and functions as a transcriptional repressor.
WTAP (Wilms tumor 1-associating protein) is a ubiquitously expressed nuclear protein that interacts with WT1 and may be involved in regulating mRNA splicing. WTAP is found in nuclear speckles, where it regulates the G2/M cell cycle transition by binding to the 3′ UTR of cyclin A2, enhancing its stability. Additionally, WTAP inhibits expression of WT1 target genes and can impair the ability of WT1 to bind DNA. Two isoforms of WTAP exist due to alternative splicing events.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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