Thermo Fisher Scientific HDAC9 Monoclonal Antibody (2B7C4)
HDAC9 단백질을 인식하는 Mouse Monoclonal Antibody (Clone 2B7C4). Western blot, ICC/IF, Flow cytometry, ELISA에 적합. Protein G로 정제된 액상 항체로, PBS buffer 및 sodium azide 함유. Human 시료에 반응하며 연구용으로 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2B7C4 |
| Immunogen | Purified recombinant fragment of human HDAC9 (AA: 343–569) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3090752 |
Target Information
In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is a critical component of transcriptional regulation, and acetylation of nucleosomal histones plays a major role in this process. Acetylation of lysine residues in histone tails induces conformational changes, increasing DNA accessibility to transcription factors.
Several mammalian proteins act as histone acetylases, including GCN5, PCAF, p300/CBP, HAT1, and TAF II p250. Conversely, histone deacetylases (HDACs) are associated with transcriptional silencing. The HDAC family includes HDAC1–9. HDAC9 and HDAC9a are alternatively spliced isoforms; HDAC9a is 132 amino acids shorter but retains the catalytic domain and deacetylase activity. Both isoforms repress myocyte enhancer-binding factor 2-mediated transcription and are expressed in brain, skeletal muscle, kidney, placenta, and pancreas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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