Thermo Fisher Scientific Phospho-IKK alpha/beta (Ser176, Ser177) Polyclonal Antibody
IKK alpha/beta 단백질의 Ser176/Ser177 인산화 상태를 특이적으로 인식하는 폴리클로날 항체입니다. Western blot, IHC, ICC/IF에 사용 가능하며, 사람, 생쥐, 랫트 시료에 반응합니다. 액상 형태로 제공되며 -20°C에서 보관합니다. 연구용 전용 제품입니다.
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- PA5104697
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- pk
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human CHUK (Accession O15111), corresponding to amino acid residues around phosphorylated Ser176 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816170 |
Product Specific Information
This antibody detects endogenous levels of IKK alpha/beta only when phosphorylated at Ser176/Ser177.
Target Information
IKK alpha/beta is encoded by CHUK (conserved helix-loop-helix ubiquitous kinase) and IKKB (inhibitor of kappa light polypeptide gene enhancer in B-cells) located on chromosomes 10 and 8, respectively, in humans. Upon stimulation by agents that activate the NF-κB pathway such as TNF alpha, the IKK complex subunits (IKK alpha, IKK beta) and regulator NEMO (IKK gamma) phosphorylate IκB proteins. This leads to ubiquitin-mediated degradation of IκB and phosphorylation of NF-κB, enabling its nuclear translocation. Phosphorylation at serine 176 and 180 in the activation loop induces a conformational change required for kinase activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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