Thermo Fisher Scientific Phospho-MEK5 (MAPKK5) (Ser311, Thr315) Polyclonal Antibody
Phospho-MEK5 (Ser311, Thr315) 특이적 폴리클로날 항체로, 인간·마우스·랫트 시료에 반응합니다. Western blot 및 IHC(P) 검증 완료. 항원 친화 크로마토그래피로 정제된 고순도 액상 항체로, EGF 자극 A431 세포에서 양성 대조로 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide surrounding the epitope -SIAKT- with double phosphorylation sites Ser311 and Thr315 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 25 mM Tris with proprietary stabilizer |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Positive control: EGF-stimulated A431 cells
- Cellular location: Cytoplasmic
Target Information
MAP2K5 (MEK5) serine/threonine kinase, also known as mitogen-activated protein kinase 5, is a dual specificity protein kinase belonging to the MAP kinase family. It specifically interacts with and activates MAPK7/ERK5. MAP2K5 can be phosphorylated and activated by MAP3K3/MEKK3 and atypical protein kinase C isoforms (aPKCs).
This signaling cascade is involved in growth factor-stimulated cell proliferation and muscle cell differentiation. The rs3743354 polymorphism in the MAP2K5 promoter may influence colorectal cancer (CRC) susceptibility.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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