Thermo Fisher Scientific CD4 Monoclonal Antibody (RM4-4), APC, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.06 µg/test
• Publications: View 3 publications

Product Specifications

Additional Formats

• Biotin
• FITC
• PE
• PE-eFluor 610
• Request custom conjugation

Product Specific Information

Description:
The RM4-4 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by most thymocytes, a subpopulation of mature T cells, and dendritic cells. CD4 binds to MHC class II on antigen-presenting cells and plays a critical role in T cell development and function. In T cells, CD4 associates with the protein tyrosine kinase Lck through its cytoplasmic tail. RM4-4 binding does not block CD4 monoclonal antibodies RM4-5 or GK1.5.

Applications Reported:
Reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤0.125 µg/test (final volume 100 µL). Optimal titration is advised per assay.

• Excitation: 633–647 nm
• Emission: 660 nm
• Laser: Red Laser

Target Information

CD4 antigen is involved in MHC class II recognition and acts as a co-receptor for HIV. It is mainly expressed on T helper lymphocytes, but also found on monocytes, macrophages, and dendritic cells. CD4 expression is observed in thymus, lymph nodes, tonsils, spleen, and various non-lymphoid tissues. It plays a role in T-cell activation via interaction with the T-cell receptor complex and Lck kinase. CD4 may also contribute to neuronal damage in infectious and immune-mediated CNS diseases. Multiple alternatively spliced transcripts have been identified (RefSeq, July 2017).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지