Thermo Fisher Scientific AKAP8 Polyclonal Antibody
AKAP8 단백질을 인식하는 Thermo Fisher Scientific의 폴리클로날 항체로, WB, IHC, ICC/IF 등 다양한 응용 가능. 인간, 생쥐, 랫트 반응성. 항원 친화 크로마토그래피로 정제된 고순도 항체. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 373–418 of human AKAP8 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2553816 |
Product Specific Information
This antibody detects endogenous AKAP8 protein at a molecular weight of approximately 95 kDa.
Purity is >95% by SDS-PAGE.
Target Information
The type II cAMP-protein kinase (PKA) is a multifunctional kinase with a broad range of substrates. Specificity of PKA signaling is mediated by compartmentalization of the kinase within the cell. The R subunit (RII) of PKA interacts with specific RII-anchoring proteins, known as A-kinase anchoring proteins (AKAPs).
AKAP95 (AKAP8) is a nuclear matrix protein predominantly expressed in liver, heart, pancreas, kidney, and skeletal muscle. During mitosis, AKAP95 is recruited to chromosomes and plays an essential role in mitotic progression. It participates in PKA signaling through interaction with the RII regulatory subunit and forms a complex with HA95 and HDAC3, required for histone H3 deacetylation during mitosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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