
Thermo Fisher Scientific K-Ras Monoclonal Antibody (9.13)
인간, 마우스, 랫트에 반응하는 K-Ras 단클론 항체로 Western blot, IHC, ICC, IP 등에 사용 가능. 단백질 A로 정제된 액상 항체이며 0.5 mg/mL 농도로 제공. PBS(pH 7.4)에 0.1% sodium azide 포함, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | View 5 publications |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:10–1:100 | – |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL | – |
| Immunoprecipitation (IP) | 5 µg | – |
| Miscellaneous PubMed (Misc) | – | View 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 9.13 |
| Immunogen | Corresponds to aa 170–180 from P01116-1 (isoform 2A) of human K-Ras |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | –20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2532192 |
Product Specific Information
This antibody is specific for human K-Ras (K-Ras2, Ki-Ras, c-K-ras, GTPase KRas) protein (accession # NP_004976.2, P01116), which is 100% homologous with mouse, 95% with rat, and 94% with bovine. Reactivity has been confirmed on Western blots with human HeLa and WI-38 cell lysates, as well as rat KNRK and mouse NIH 3T3 cell lysates, identifying the target band at approximately 21 kDa. Reactivity has also been confirmed with rat KNRK cells using immunoprecipitation and with HeLa cells by immunofluorescence. Based on amino acid sequence homology, reactivity with bovine is also expected.
Target Information
K-RAS (GTPase KRas) belongs to the Ras oncogene family. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. It plays a key role in regulating cell proliferation and contributes to oncogenic processes by inducing transcriptional silencing of tumor suppressor genes in colorectal cancer cells in a ZNF304-dependent manner. Mutations in this gene are associated with acute myelogenous leukemia, juvenile myelomonocytic leukemia, Noonan syndrome, gastric cancer, and cardiofaciocutaneous syndrome 2.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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