Thermo Fisher Scientific Mouse Fc gamma RI (CD64) His-tag Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (IHC)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Functional Assay (Functional)

Assay-dependent

-

Product Specifications

Species

Mouse

Expression System

HEK293 cells

Amino acid sequence

Mouse FCGR1, amino acids Met1-Pro297 (Accession # NP_034316.1) with a C-terminal His-tag

Tag

His-tag

Molecular weight

32 kDa

Class

Recombinant

Type

Protein

Purity

>95 % as determined by SDS-PAGE. >90 % as determined by SEC-HPLC

Endotoxin concentration

<1.0 EU/µg

Activity

1.Immobilized Rituximab (IgG1) at 1 µg/mL can bind Mouse Fc gamma RI (CD64) His-tag Recombinant Protein, the EC50 is 16-35 ng/mL.
2.Immobilized Anti-CD3 antibody, mouse IgG2a at 2 µg/mL (100 µL/well) can bind Mouse Fc gamma RI (CD64) His-tag Recombinant Protein, the EC50 is 0.8-2.4 ng/mL(QC tested).
3.Loaded Mouse Fc gamma RI (CD64) His-tag Recombinant Protein on His1K Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 0.12 µM as determined in a BLI assay (Sartorius Octet Red384) (Routinely tested).
4.Loaded Mouse Fc gamma RI (CD64) His-tag Recombinant Protein on His1K Biosensor, can bind Nivolumab (IgG4) with an affinity constant of 0.28 µM as determined in a BLI assay (Sartorius Octet Red384) (Routinely tested).
5.Captured Mouse Fc gamma RI (CD64) His-tag Recombinant Protein on Anti-His Chip can bind Bevacizumab (IgG1) with an affinity constant of 0.12 µM as determined in an SPR assay (Biacore T200) (Routinely tested).
6.Captured Mouse Fc gamma RI (CD64) His-tag Recombinant Protein on Anti-His Chip can bind Nivolumab (IgG4) with an affinity constant of 0.69 µM as determined in an SPR assay (Biacore T200) (Routinely tested).
7.The purity of Mouse Fc gamma RI (CD64) His-tag Recombinant Protein is more than 95% and the molecular weight of this protein is around 40-50 kDa verified by SEC-MALS.
8.Loaded Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (OKT3) on ProA Biosensor, can bind Mouse Fc gamma RI (CD64) His-tag Recombinant Protein with an affinity constant of 3.46 nM as determined in BLI assay (Sartorius Octet RED384)(QC tested).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Storage buffer

PBS, pH 7.4, with 5% mannitol, 5% trehalose, 0.01% Tween 80

Contains

no preservative

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient

Product Specific Information

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Target Information

CD64 (FCGR1A) encodes a protein that plays an important role in the immune response. CD64 is a high-affinity Fc-gamma receptor and is one of three related gene family members located on chromosome 1. CD64 plays an important role in the immune response and its dysfunction has been studied in cervical adenitis and tetrasomy 21.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.