Thermo Fisher Scientific CD22 Monoclonal Antibody (OTI2E6), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:150

Product Specifications

Product Specific Information

• For reconstitution, add 100 µL distilled water to achieve a final concentration of approximately 1 mg/mL.
• For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

CD22 (BL-CAM) is a type 1 integral membrane glycoprotein (130–140 kDa) expressed in both the cytoplasm and membrane of B-lymphocytes.
It appears early in B-cell differentiation, similar to CD19, and is mainly expressed in mature B cells but absent in plasma cells.
CD22 is strongly expressed in hairy cell leukemia and is useful for phenotyping mature leukemias.
It binds preferentially to α2,6-linked sialic acid, with its recognition site modulated by cis interactions.
Upon ligand-induced tyrosine phosphorylation, CD22 participates in regulation of B-cell receptor signaling, acting as both a positive and inhibitory regulator through interactions with Src family kinases and SH2 domain-containing phosphatases.
CD22 is positive in diffuse large B-cell lymphoma and nodular lymphocyte predominance Hodgkin’s lymphoma, but negative in classical Hodgkin’s lymphoma.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.