Thermo Fisher Scientific CD39 Monoclonal Antibody (eBioA1 (A1)), PerCP-eFluor 710, eBioscience
인간 CD39(ENTPD1)를 인식하는 eBioA1 단클론 항체로, PerCP-eFluor 710 형광 표지. Flow cytometry에 최적화되어 있으며, 5 µL/test로 사용. Treg 및 면역세포의 CD39 발현 분석에 적합. 4°C 암소 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (Frozen) (IHC-F) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.06 µg)/test | View 14 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-eFluor™ 710, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioA1 (A1) |
| Conjugate | PerCP-eFluor™ 710 |
| Excitation/Emission Max | 482 / 708 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10598507 |
Available Formats
- Unconjugated
- APC
- APC-eFluor 780
- Brilliant UV 805
- FITC
- NovaFluor Blue 610-70S
- NovaFluor Blue 660-120S
- NovaFluor Blue 690
- NovaFluor Red 700
- NovaFluor Red 725
- NovaFluor Red 755
- NovaFluor Yellow 660
- NovaFluor Yellow 755
- PE
- PE-Cyanine7
- Super Bright 436
- Super Bright 600
- Super Bright 702
- Request custom conjugation
Product Specific Information
The eBioA1 monoclonal antibody reacts with human CD39 (ENTPD1 or NTPDase), an integral membrane protein with two transmembrane domains forming a homotetramer. CD39 functions to remove extracellular ATP by converting it to ADP or AMP, often working with CD73. Expression is found on activated lymphocytes, subsets of T cells, B cells, dendritic cells, and weakly on monocytes and granulocytes.
CD39 and CD73 are also expressed on regulatory T cells (Treg), facilitating their migration into inflamed tissues with high ATP levels. Expression of CD39 on Foxp3+CD4+ cells ranges from 25–45%.
Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated for flow cytometry of human peripheral blood cells. Use 5 µL (0.06 µg) per test (100 µL final volume). Cell count: 10⁵–10⁸ cells/test.
Fluorochrome: PerCP-eFluor® 710 (Emission 710 nm, excitation 488 nm).
Recommended filter: 710/50 bandpass (695/40 acceptable).
Ensure instrument compatibility.
Fixation:
Samples can be stored in:
- IC Fixation Buffer (Product #00-8222)
- 1-step Fix/Lyse Solution (Product #00-5333)
Store up to 3 days at 4°C in the dark with minimal signal loss.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD39 (70–100 kDa) is expressed on peripheral blood B cells, monocytes, T cell clones, and weakly on granulocytes. It has ecto-ATPase activity and is upregulated on T and B cells upon activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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