Thermo Fisher Scientific Goat anti-Human IgE Secondary Antibody, FITC
Goat anti-Human IgE FITC 결합 2차 항체로, ICC/IF 및 유세포분석에 적합합니다. Human IgE에 특이적으로 반응하며, 높은 민감도와 신뢰성을 제공합니다. 형광 검출용으로 498/517 nm 파장에서 최적의 신호를 제공합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 1:500–1:20,000 |
| Flow Cytometry (Flow) | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Human IgE |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.8, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535571 |
| Target | IgE |
| Antibody Form | Whole Antibody |
Additional Formats
- AP
- Biotin
- HRP
- TRITC
- Request custom conjugation
Product Specific Information
The sensitivity of each lot of antibody is confirmed using ELISA.
The specificity of each lot of antibody is confirmed by immunoelectrophoresis (IEP).
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting, or purification of their specified targets.
Secondary antibodies offer increased versatility, enabling users to use many detection systems (e.g., HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e., immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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