Thermo Fisher Scientific Goat anti-Human IgE Secondary Antibody, Biotin
Goat anti-Human IgE Biotin 결합 2차 항체로, ELISA, ICC/IF, Flow Cytometry 등에 사용. Human IgE에 특이적이며, 높은 민감도와 신뢰성 제공. Affinity chromatography로 정제되어 낮은 background 보장. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 1:500–1:20,000 |
| Flow Cytometry (Flow) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Human IgE |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535574 |
| Target | IgE |
| Antibody Form | Whole Antibody |
Additional Formats
Product Specific Information
Each lot of antibody is tested for sensitivity using ELISA and for specificity by immunoelectrophoresis (IEP), ensuring consistent performance.
Target Information
Anti-Human secondary antibodies are affinity-purified with well-characterized specificity for human immunoglobulins. They are useful for detection, sorting, or purification of specific targets.
Secondary antibodies enhance versatility and sensitivity by allowing multiple detection systems (e.g., HRP, AP, fluorescence) and signal amplification, as multiple secondary antibodies can bind to one primary antibody.
Typically, these antibodies are generated by immunizing the host animal with pooled human immunoglobulins and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, label conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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