
Thermo Fisher Scientific Goat anti-Human IgE Secondary Antibody, HRP
Goat anti-Human IgE HRP 결합 2차 항체로 Western blot, IHC, ELISA 등 다양한 면역분석에 사용 가능. 친화 크로마토그래피로 정제되어 높은 특이성과 감도를 제공. 동결건조 형태로 제공되며 재구성 후 장기 보관 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Human IgE |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.1% ProClin 150 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2535570 |
| Target | IgE |
| Antibody Form | Whole Antibody |
Additional Formats
- AP conjugate
- Biotin conjugate
- FITC conjugate
- TRITC conjugate
- Custom conjugation available upon request
Product Specific Information
Each lot of antibody is tested for sensitivity using ELISA and for specificity using immunoelectrophoresis (IEP).
Rehydrate with 1.1 mL of deionized water and allow to stand for 30 minutes at room temperature to dissolve. (Product is overfilled to ensure complete recovery.) Centrifuge to remove particulates. Prepare fresh working dilution daily.
Store lyophilized material at 2–8°C. For long-term storage after reconstitution, dilute with 50% glycerol and store at -20°C as a liquid.
Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin human serum proteins or light chains on all human immunoglobulins.
Target Information
Anti-Human secondary antibodies are affinity-purified and highly specific for human immunoglobulins. They are useful for detection, sorting, or purification of their target.
Secondary antibodies enable multiple detection systems (e.g., HRP, AP, fluorescence) and provide signal amplification since multiple secondary antibodies can bind to a single primary antibody.
These antibodies are typically generated by immunizing the host animal with pooled human immunoglobulins and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, conjugation) for high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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