
Thermo Fisher Scientific Goat anti-Human IgE Secondary Antibody
Goat에서 유래한 anti-Human IgE 2차 항체로, Western blot과 ELISA에 적합합니다. Affinity chromatography로 정제된 액상 항체이며, 높은 특이성과 민감도를 제공합니다. 비결합(Unconjugated) 상태로 다양한 검출 시스템에 활용 가능합니다.
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Applications and Tested Dilution
- Western Blot (WB): 1:2000
- ELISA: 1:500–1:20,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Human IgE |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535572 |
| Target | IgE |
| Antibody Form | Whole Antibody |
Product Specific Information
The sensitivity of each lot of antibody is confirmed using ELISA.
The specificity of each lot of antibody is confirmed by immunoelectrophoresis (IEP).
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting, or purification of their specified targets.
Secondary antibodies offer increased versatility, enabling users to employ various detection systems (e.g., HRP, AP, fluorescence). They can also enhance sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and may be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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