Thermo Fisher Scientific SHC Monoclonal Antibody (2F7C7)
SHC 단백질을 표적으로 하는 Thermo Fisher Scientific의 2F7C7 마우스 단클론 항체. Western blot, ICC/IF, Flow cytometry, ELISA에 적합. 고순도 Protein G 정제, 1 mg/mL 농도, PBS buffer 및 sodium azide 포함. 연구용으로만 사용.
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Thermo Fisher Scientific SHC Monoclonal Antibody (2F7C7)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2F7C7 |
| Immunogen | Purified recombinant fragment of human SHC1 (aa 385–495) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2898543 |
Product Specific Information
This antibody has been tested in indirect ELISA.
Target Information
Skp2 (S-phase Kinase-associated Protein 2) belongs to the family of F-box proteins that interact with the Cyclin A-Cdk2 complex. Skp2 is essential for the G1-S transition in both transformed cells and diploid fibroblasts. It is required for the ubiquitination and degradation of p27 in mammalian cells. In normal tissues, Skp2 is expressed in tonsil and placenta, while in tumor tissues it shows wide expression, including colon, prostate, pancreas, and skin cancers, with high levels in lung, breast, ovarian, and endometrial cancers. Research suggests Skp2 plays a role in oncogenesis, particularly in lymphomas and breast cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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