Thermo Fisher Scientific SETD7 Monoclonal Antibody (3D6G11)
SETD7 단백질을 인식하는 Mouse monoclonal 항체로, WB, IHC(P), ELISA에 사용 가능. Human, Rat, Non-human primate 반응성. Protein G 정제, 1 mg/mL 농도. Epigenetic 연구 및 전사 조절 기전 분석에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| ELISA | 1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Non-human primate, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3D6G11 |
| Immunogen | Purified recombinant fragment of human SETD7 (aa: 107–366) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2898541 |
Product Specific Information
This antibody has been tested in indirect ELISA.
Target Information
Histone methyltransferase that specifically monomethylates Lys-4 of histone H3. H3 Lys-4 methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activation of transcription.
Also exhibits methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K motif in substrate proteins.
Monomethylates Lys-189 of TAF10, increasing its affinity for RNA polymerase II, and Lys-372 of p53/TP53, stabilizing p53/TP53 and enhancing transcriptional activation. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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