Thermo Fisher Scientific IFN gamma Monoclonal Antibody (4S.B3), Brilliant Ultra Violet 737, eBioscience

Thermo Fisher Scientific IFN gamma Monoclonal Antibody (4S.B3), Brilliant Ultra Violet 737, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Intracellular staining)
• Tested Dilution: 5 µL (0.25 µg)/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 615, 661, 805
• Brilliant Violet 650
• eFluor 450, 506, 660
• FITC, PE, PE-Cyanine7, PE-eFluor 610, PerCP-Cyanine5.5
• Request custom conjugation

Product Specific Information

The 4S.B3 monoclonal antibody reacts with human interferon-gamma (IFN-γ), a 17 kDa cytokine produced by activated T and NK cells with antiviral and antiparasitic properties. IFN-γ acts synergistically with TNF-α to inhibit cell proliferation and modulate immune responses across various cell types.

• Applications Reported: Intracellular staining followed by flow cytometric analysis.
• Applications Tested: Pre-diluted and validated using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00).
  Recommended usage is 5 µL (0.25 µg) per test (100 µL final volume).
  Cell count: 10⁵–10⁸ cells/test (empirically determined).

Brilliant Ultra Violet™ 737 (BUV737) emits at 732 nm and is optimized for use on cytometers equipped with a 355 nm UV laser. Ensure instrument compatibility before use.

• Buffer Recommendations: When using multiple polymer dyes, use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer (Product # 00-4409) to minimize non-specific interactions.
• Light Sensitivity: Protect from light to prevent photo-induced oxidation.
• Fixation: Compatible with IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark.
• Methanol Compatibility: Suitable for short-term methanol-based fixation (<1 hour).
• Excitation/Emission: 355 nm / 732 nm; Laser: Ultraviolet.

Trademark Notice: BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

IFN-γ (Interferon gamma, Type II interferon) is a macrophage activation factor produced mainly by T-lymphocytes and NK cells. It is a 45 kDa homodimeric glycoprotein composed of two 146 amino acid subunits. IFN-γ exhibits species-specific activity and does not cross-react between human and mouse.

Functions include:

• Antiviral and antiproliferative activity
• Induction of MHC class I and II expression
• Macrophage activation
• Enhancement of immunoglobulin secretion by B cells
• Cytokine regulation via JAK-STAT pathway activation

Human IFN-γ shows ~40% sequence homology with mouse IFN-γ. Mutations in the IFN-γ gene are associated with aplastic anemia.

For Research Use Only. Not for diagnostic procedures or resale without authorization.

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