Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (J43), Brilliant Ultra Violet 737, eBioscience

Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (J43), Brilliant Ultra Violet 737, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry
• Tested Dilution: 0.5 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 563 / 615 / 661 / 805
• Brilliant Violet 480 / 605 / 650 / 786
• eFluor 450 / 506
• FITC
• Functional Grade
• PE / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 702 / 780
• Custom conjugation available

Product Specific Information

The J43 monoclonal antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily. PD-1 plays a critical role in peripheral tolerance and autoimmune disease in mice and is mainly expressed on activated T and B lymphocytes.
J43 inhibits the binding of mouse PD-L1-Ig and PD-L2-Ig to PD-1/BHK transfected cells and has been shown to modulate immune responses in vivo.

Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of stimulated mouse splenocytes at ≤0.5 µg/test (100 µL final volume, 10⁵–10⁸ cells/test).
Optimize antibody concentration for best performance.

Fluorophore Information:
Brilliant Ultra Violet™ 737 (BUV737) emits at 732 nm and is designed for UV (355 nm) laser-equipped cytometers. Protect from light due to photo-induced oxidation sensitivity.

Fixation Compatibility:
Samples can be stored up to 3 days in the dark at 4°C using IC Fixation Buffer or 1-step Fix/Lyse Solution. Compatible with short-term methanol-based fixation (≤1 hour).

Recommended Buffers:

• Super Bright Complete Staining Buffer (SB-4401)
• Brilliant Stain Buffer (00-4409)

Laser Settings:
Excitation: 355 nm
Emission: 732 nm
Laser: Ultraviolet

Trademark Notice:
BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates. Powered by Sirigen™.

Target Information

PD-1 (PDCD-1) is an inhibitory receptor expressed on activated T cells. It binds PD-L1 and PD-L2 on antigen-presenting cells to downregulate immune responses. In PD-1 deficient mice, hyperactivated immune systems and autoimmune diseases are observed.
Although PD-1 has a predicted molecular weight of 55 kDa, it may migrate at higher weight in SDS-PAGE.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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