Thermo Fisher Scientific CD235a (Glycophorin A) Monoclonal Antibody (10F7MN), Brilliant Ultra Violet 737, eBioscience
인체 적혈구 표면의 CD235a(Glycophorin A)를 인식하는 10F7MN 단클론 항체로, Brilliant Ultra Violet 737 형광체 결합. Flow Cytometry 분석에 최적화되어 있으며, M/N 알렐 모두 검출 가능. 광민감성 주의 및 4°C 암소 보관 권장.
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Thermo Fisher Scientific CD235a (Glycophorin A) Monoclonal Antibody (10F7MN), Brilliant Ultra Violet 737, eBioscience
Applications and Tested Dilution
- Application: Flow Cytometry
- Tested Dilution: 5 µL (0.25 µg)/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Brilliant Ultra Violet™ 737 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 10F7MN |
| Conjugate | Brilliant Ultra Violet™ 737 |
| Excitation / Emission Max | 350 / 740 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2896055 |
Additional Formats
- Brilliant UV 661
- FITC
- NovaFluor Blue 510
- NovaFluor Blue 585
- Request custom conjugation
Product Specific Information
The monoclonal antibody 10F7MN recognizes human glycophorin A (CD235a), detecting both M and N alleles. Glycophorin A is a 151 amino acid sialoglycoprotein expressed on erythrocyte and erythroid progenitor membranes (~500,000 copies per cell). The gene resides on chromosome 4 with two allelic forms (M and N), differing by two amino acids. Studies link genotype/phenotype variations with disease predisposition.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-diluted; use at 5 µL (0.25 µg)/test in 100 µL final volume (10⁵–10⁸ cells/test)
Brilliant Ultra Violet™ 737 (BUV737) emits at 732 nm and is designed for UV (355 nm) laser-equipped cytometers. Ensure instrument compatibility.
When using multiple polymer dye-conjugated antibodies (Super Bright™, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (SB-4401) or Brilliant Stain Buffer (00-4409) to minimize non-specific interactions.
Light Sensitivity: Protect vial and stained samples from light.
Fixation: Samples may be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (00-8222) or 1-step Fix/Lyse Solution (00-5333).
Compatibility: Short-term methanol fixation is acceptable; avoid prolonged methanol exposure (>1 hour).
Excitation: 355 nm
Emission: 732 nm
Laser: Ultraviolet Laser
BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.
Target Information
CD235a (Glycophorin A, GPA) is a membrane sialoglycoprotein expressed in mature erythrocytes and erythroid precursors. It carries M/N blood group specificities and may act as a barrier to cell fusion, reducing RBC aggregation. CD235a also serves as a receptor for Sandei virus, parvovirus, and Streptococcus adhesin Hsa.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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