Himedia HiSep LSM 1084

It is based on the adapted method of isolating human mononuclear cells using centrifugation techniques by Bøyum in which defibrinated blood is layered on a solution of sodium diatrizoate and polysucrose and centrifuged at low speeds for 30 minutes. Differential migration following centrifugation results in the formation of several cell layers. Mononuclear cells (lymphocytes and monocytes) and platelets are contained in the banded plasma-LSM interphase due to their density, and the pellet that is formed contains mostly erythrocytes and granulocytes, which have migrated through the gradient to the bottom of the tube. HiSepTM LSM 1084 is a solution of polysucrose and sodium diatrizoate, adjusted to a density of 1.084± 0.0010g/ml. This medium offers a quick and reliable method for the simple isolation of mononuclear cells from blood from chicken, rats, mice and other mammals.