
Thermo Fisher Scientific Rat IgG1 kappa Isotype Control (eBRG1), Alexa Fluor 488, eBioscience
Alexa Fluor 488 형광으로 표지된 Rat IgG1 kappa 동형 대조 항체. IHC, ICC/IF, Flow Cytometry 등에서 음성 대조용으로 사용. 499/520 nm 파장 특성. 4°C 암소 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (IHC) | Assay-Dependent |
| Immunocytochemistry (ICC/IF) | Assay-Dependent |
| Flow Cytometry (Flow) | Assay-Dependent |
| Control (Ctrl) | Assay-Dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host/Isotype | Rat / IgG1, kappa |
| Class | Monoclonal |
| Type | Isotype Control |
| Clone | eBRG1 |
| Conjugate | Alexa Fluor™ 488 |
| Excitation/Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_493962 |
Additional Available Formats
Unconjugated, Alexa Fluor 532, Alexa Fluor 561, Alexa Fluor 647, Alexa Fluor 660, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV 395, Brilliant UV 563, Brilliant UV 615, Brilliant UV 661, Brilliant UV 737, Brilliant UV 805, Brilliant Violet 421, Brilliant Violet 480, Brilliant Violet 605, Brilliant Violet 650, Brilliant Violet 711, Brilliant Violet 786, eFluor 450, eFluor 506, eFluor 570, eFluor 615, eFluor 660, FITC, Functional Grade, Pacific Orange, PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610, PerCP-Cyanine5.5, PerCP-eFluor 710, Super Bright 436, Super Bright 600, Super Bright 645, Super Bright 702, Super Bright 780.
Product Specific Information
- Description: Monoclonal rat IgG1, kappa is useful as an isotype control immunoglobulin.
- Applications Reported: Alexa Fluor® 488 Rat IgG1 K Isotype Control is used in immunohistochemistry, immunocytochemistry, and flow cytometric analysis.
- Applications Tested: Used for flow cytometric analysis of mouse splenocyte suspensions; apply at the same concentration as the experimental antibody.
- Excitation/Emission: 488 nm / 519 nm; Laser: Blue Laser.
- Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Rat IgG1 is a subclass of immunoglobulin G (IgG) antibodies commonly used in research and diagnostics. These antibodies, produced by rats in response to antigens, can be purified from serum or hybridoma culture supernatants. Rat IgG1 is often used as a negative control since it does not bind to most mammalian antigens. It is applicable in immunohistochemistry, flow cytometry, and western blotting to detect specific antigens. Gene targeting using rat models enables the study of gene function and disease mechanisms by allowing gene knockout, insertion, or modification.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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