Thermo Fisher Scientific DOCK3 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:500

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human DOCK3. Recombinant protein control fragment (Product #RP-96295). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:DOCK3,uniProtId:Q8IZD9-1,ncbiNodeId:9606,antigenRange:167-260,antigenLength:2030,antigenImageFileName:PA5-57996_DOCK3_Q8IZD9-1_Rabbit.svg,antigenImageFileNamePDP:PA5-57996_DOCK3_Q8IZD9-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.4 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2640738

Product Specific Information

Immunogen sequence: FEVVDSDQIS VSDLYKMHLS SRQSVQQSTS QVDTMRPRHG ETCRMPVPHH FFLSLKSFTY NTIGEDTDVF FSLYDMREGK QISERFLVRL NKNG

Highest antigen sequence identity to the following orthologs: Mouse - 98%, Rat - 98%.

Target Information

Dedicator of cytokinesis3 (DOCK3), also named as MOCA and PBP, is a ~180 kDa protein involved in signaling transduction. It is a potential guanine nucleotide exchange factor (GEF) which activate some small GTPases by exchanging bound GDP for free GTP. DOCK3 is associated in Alzheimer disease tangles and regulates the accumulation of amyloid precursor protein and beta-amyloid. Overexpression of Dock3 in neural cells promotes axonal outgrowth downstream of brain-derived neurotrophic factor (BDNF) signaling. DOCK3 binds to and inactivates glycogen synthase kinase-3beta (GSK-3beta) at the plasma membrane, thereby promoting axon branching and microtubule assembly. By stimulating actin polymerization and microtubule assembly, DOCK3 plays important roles downstream of BDNF signaling in the CNS.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.