Thermo Fisher Scientific SELK Polyclonal Antibody
SELK 단백질을 인식하는 Rabbit Polyclonal 항체로, WB 및 IHC에 사용 가능. 인간, 마우스, 랫트 반응성. 항원 친화 크로마토그래피로 정제된 액상 항체로, 1 mg/mL 농도. 심장과 면역세포에서의 SELK 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | View 1 publication |
| Immunohistochemistry (IHC) | 20 µg/mL | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | An 18 amino acid synthetic peptide near the carboxy terminus of human SELK |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long term storage, store at –20°C. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2551772 |
Product Specific Information
- A suggested positive control is A-20 cell lysate.
- PA5-34420 can be used with blocking peptide PEP-1462.
Target Information
SELK is a selenoprotein containing a selenocysteine residue at its active site. It is localized to the endoplasmic reticulum and highly expressed in the heart, where it may function as an antioxidant. SELK interacts with ER-associated degradation (ERAD) components such as p97 ATPase, Derlin-1 and -2, and SelS, as well as proteins in the oligosaccharyltransferase complex. These interactions suggest SELK’s involvement in Derlin-dependent ERAD of glycosylated misfolded proteins.
SELK also plays a role in Ca²⁺ flux in immune cells and is cleaved by m-calpain in resting macrophages; this cleavage is inhibited upon activation through Toll-like receptors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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