Thermo Fisher Scientific DDX60 Monoclonal Antibody (1A10)
인간 DDX60 단백질을 인식하는 Mouse IgG1 단클론 항체. Western blot 및 ELISA에 사용 가능. GST 태그가 포함된 전장 재조합 단백질로 면역원 제작. 연구용으로만 사용, 진단용 불가.
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Applications
Western Blot (WB)
- Tested Dilution: 1–5 µg/mL
ELISA
- Tested Dilution: 1 ng/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1A10 |
| Immunogen | DDX60 (AAH20601.1, 1 a.a. ~183 a.a) full-length recombinant protein with GST tag (GST tag MW: 26 KDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein is as follows:
MKIMEDFTTF LRIVSKLADM NQEYQLPLSK IKFTGKECED SQLVSHLMSC KEGRVAISPF VCLSGNFDDD LLRLETPNHV TLGTIGVNRS QAPVLLSQKF DNRGRKMSLN AYALDFYKHG SLIGLVQDNR MNEGDAYYLL KDFALTIKSI SVSLRELCEN EDDNVVLAFE QLSTTFWEKL NKV
Target Information
DDX60 is a DEXD/H Box RNA helicase and the human homolog of the S. cerevisiae Ski2, a cofactor of RNA exosome. Expression of DDX60 increases after viral infection and binds to endogenous RIG-I, a cytoplasmic viral RNA sensor, as well as other RIG-I-like receptors such as MDA5 and LGP2. These proteins activate TBK1 and IKK-e, signaling IRF3 and NF-kB-responsive genes in the innate immune response pathway. DDX60 is required for the RIG-I- or MDA5-dependent response to viral infection, but is dispensable for TLR3-mediated signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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