Thermo Fisher Scientific DDX60 Monoclonal Antibody (1A10)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

-

ELISA (ELISA)

1 ng/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

1A10

Immunogen

DDX60 (AAH20601.1, 1 a.a. approximately 183 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:DDX60,uniProtId:Q8IY21-1,ncbiNodeId:9606,antigenRange:1-1712,antigenLength:1712,antigenImageFileName:H00055601-M02_DDX60_Q8IY21-1_House_mouse.svg,antigenImageFileNamePDP:H00055601-M02_DDX60_Q8IY21-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Sequence of this protein is as follows: MKIMEDFTTF LRIVSKLADM NQEYQLPLSK IKFTGKECED SQLVSHLMSC KEGRVAISPF VCLSGNFDDD LLRLETPNHV TLGTIGVNRS QAPVLLSQKF DNRGRKMSLN AYALDFYKHG SLIGLVQDNR MNEGDAYYLL KDFALTIKSI SVSLRELCEN EDDNVVLAFE QLSTTFWEKL NKV

Target Information

DDX60 is a DEXD/H Box RNA helicase and the human homolog of the S. cerevisiae Ski2, a cofactor of RNA exosome. Expression of DDX60 increases after viral infection and binds to endogenous RIG-I, a cytoplasmic viral RNA sensor, as well as other RIG-I-like receptors such as MDA5 and LGP2. These proteins activate TBK1 and IKK-e, signaling IRF3 and NF-kB-responsive genes in the innate immune response pathway. DDX60 is required for the RIG-I- or MDA5-dependent response to viral infection, but is dispensable for TLR3-mediated signaling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.