Thermo Fisher Scientific Goat anti-Human IgG Fc Secondary Antibody, Biotin
Goat 유래 Biotin 결합형 anti-Human IgG Fc 2차 항체로, ELISA, Western blot, IHC, Flow cytometry 등에 사용 가능. 높은 특이성과 감도로 신뢰성 있는 검출 및 신호 증폭 제공. Affinity chromatography로 정제되어 안정적인 성능 유지.
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Thermo Fisher Scientific Goat anti-Human IgG Fc Secondary Antibody, Biotin
Applications 및 권장 희석 배수
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:20,000–1:500,000 |
| Immunohistochemistry (IHC) | 1:500–1:5,000 |
| Flow Cytometry (Flow) | 1:500–1:20,000 |
| ELISA | 1:20,000–1:500,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobulin Fc region |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535598 |
| Target | IgG Fc |
| Antibody Form | Whole Antibody |
Product Specific Information
Each lot of this antibody is tested for sensitivity using ELISA and specificity confirmed by immunoelectrophoresis (IEP).
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins. They are useful for detection, sorting, or purification of the specified target. Secondary antibodies provide versatility for multiple detection systems (HRP, AP, fluorescence) and enhanced sensitivity through signal amplification. Typically, they are generated by immunizing the host animal with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, label conjugation) for high specificity.
Additional Formats
- Alexa Fluor 488
- Alexa Fluor 647
- Alexa Fluor Plus 488
- Alexa Fluor Plus 647
- AP
- FITC
- HRP
- PE
- TRITC
- Custom conjugation available upon request
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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