Thermo Fisher Scientific Goat anti-Human IgG Fc Secondary Antibody, AP
Goat anti-Human IgG Fc AP 결합 2차 항체로, Western blot, IHC, ELISA 등 다양한 면역 분석에 사용됩니다. 고순도 친화성 정제 항체로 높은 특이성과 감도를 제공합니다. Human IgG Fc 영역에 특이적으로 결합하며, 연구용으로 적합합니다.
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Applications and Tested Dilution
| Application | Dilution Range |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobin Fc region |
| Conjugate | AP |
| Form | Liquid |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 30 mM triethanolamine, 1% BSA, 5 mM MgCl₂, 0.1 mM ZnCl₂ |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2535597 |
| Target | IgG Fc |
| Antibody Form | Whole Antibody |
Additional Formats Available
- Alexa Fluor 488
- Alexa Fluor 647
- Alexa Fluor Plus 488
- Alexa Fluor Plus 647
- Biotin
- FITC
- HRP
- PE
- TRITC
- Custom conjugation available upon request
Product Specific Information
Each lot is tested for sensitivity using ELISA and for specificity using immunoelectrophoresis (IEP).
Target Information
Anti-Human secondary antibodies are affinity-purified and exhibit well-characterized specificity for human immunoglobulins. They are widely used for detection, sorting, or purification of human antibodies.
Secondary antibodies enhance assay flexibility and sensitivity by enabling multiple detection systems (HRP, AP, fluorescence) and signal amplification.
These antibodies are typically generated by immunizing host animals with pooled immunoglobulins from the target species and further purified or modified to achieve high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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