
Thermo Fisher Scientific Goat anti-Human IgG Fc Secondary Antibody, HRP
HRP 결합형 염소 유래 항-인간 IgG Fc 2차 항체로, Western blot, IHC, ELISA에 적합합니다. 고감도·특이성 검증 완료, 친화 크로마토그래피 정제, 안정적 장기보관 가능. 연구용으로만 사용됩니다.
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Thermo Fisher Scientific Goat anti-Human IgG Fc Secondary Antibody, HRP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobulin Fc region |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.1% ProClin 150 |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2535594 |
| Target | IgG Fc |
| Antibody Form | Whole Antibody |
Available Conjugate Formats
- Alexa Fluor 488
- Alexa Fluor 647
- Alexa Fluor Plus 488
- Alexa Fluor Plus 647
- AP
- Biotin
- FITC
- PE
- TRITC
- Request custom conjugation available
Product Specific Information
- Each lot is tested for sensitivity using ELISA and specificity using immunoelectrophoresis (IEP).
- Rehydrate with 1.1 mL deionized water and let stand 30 minutes at room temperature to dissolve.
- Centrifuge to remove particulates and prepare fresh working dilution daily.
- Store lyophilized material at 2–8°C.
- For long-term storage after reconstitution, dilute with 50% glycerol and store at -20°C as liquid.
- Based on IEP, no reactivity is observed to non-immunoglobulin human serum proteins or light chains of human immunoglobulins.
Target Information
Anti-Human secondary antibodies are affinity-purified with well-characterized specificity for human immunoglobulins.
They are useful for detection, sorting, or purification of human antibody targets.
Secondary antibodies enhance versatility and sensitivity via multiple detection systems (HRP, AP, fluorescence) and signal amplification.
Typically generated by immunizing host animals with pooled immunoglobulins from the target species, then purified and modified (e.g., immunoaffinity chromatography, fragmentation, conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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