Thermo Fisher Scientific MFN1 Monoclonal Antibody (OTI3C5), TrueMAB
Human MFN1 단백질(aa 209–469)에 특이적인 Mouse IgG1 단클론 항체로, Western Blot에 최적화됨. 동결건조 형태로 제공되며, PBS/trehalose buffer에 보존. 미토콘드리아 융합 및 Bax 활성 조절 연구에 적합한 연구용 항체.
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Applications
- Western Blot (WB)
Tested Dilution: 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3C5 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 209–469 of human MFN1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Mitofusin 1 (MFN1) and the related protein MFN2 are mitochondrial membrane GTPase proteins that play a key role in mitochondrial metabolism. They may be associated with obesity and apoptosis processes.
MFN1 and MFN2 form homotypic and heterotypic complexes, regulating mitochondrial fusion and being essential for embryonic development. When ectopically expressed, MFN1 inhibits the apoptosis-associated conformational change in Bax but not its mitochondrial translocation, suggesting MFN1 regulates Bax activation on the outer mitochondrial membrane.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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